IB Project details
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| IB Project: Membrane specificity and Organelle Biogenesis | ||
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| Faculty: Medicine - UMCU | Department: Cell Biology | Click to see website |
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| IB Staff:
(IB contact/IB board) Prof.dr. Judith Klumperman j.klumperman@umcutrecht.nl Tel: +31 (0)88 755 6650 |
PhD Student(s): MSc. Romain Galmes r.galmes@umcutrecht.nl Drs. Eline van Meel h.m.m.vanmeel@umcutrecht.nl MSc. Maaike Pols M.S.Pols-2@umcutrecht.nl |
Postdoc(s): Dr. Ann de Mazière a.m.g.l.demaziere@umcutrecht.nl Dr. Carolien van Rijnsoever C.vanrijnsoever@umcutrecht.nl |
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| IB Staff:
Dr. Catherine Rabouille c.rabouille@umcutrecht.nl Tel: +31 (0)88 755 9280 |
Postdoc(s): Dr. Bram Herpers b.h.a.herpers@umcutrecht.nl Dr. Viorica Ivan V.Ivan@umcutrecht.nl Dr. Gert de Voer g.devoer@umcutrecht.nl |
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| IB Staff:
Dr. Fulvio Reggiori f.reggiori@umcutrecht.nl Tel: +31 (0)88 755 6551 |
PhD Student(s): MSc. Ester Rieter e.m.rieter@umcutrecht.nl Drs. Mustafa Ulasli m.ulasli@umcutrecht.nl Drs. Aniek van der Vaart a.vandervaart-2@umcutrecht.nl |
Postdoc(s): Dr. Eduardo Cebollero e.cebollero@umcutrecht.nl Dr. Muriel Mari M.Mari@umcutrecht.nl |
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| Available techniques, facilities and expertise: 1 3D tomography |
Contact and or Remarks: |
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| 2 Confocal microscopy | ||
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| 3 Correlative live cell-immuno electron microscopy (CLEM) Advantage: combines advantages of LM and EM | ||
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4 Drosophila facility (D2 level for genetically modified organisms)
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Dr. Catherine Rabouille c.rabouille@umcutrecht.nl Fly work, stocks, crosses. Please contact me for availability as the suite is small. | |
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| 5 Electron microscopy | ||
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| 6 EM tomography | To generate 3D images of cellular membrane structures. Cell Biology UMCU / Molecular Cell Biology. | |
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| 7 Fluorescence microscopy | ||
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| 8 FRET-FLIM: study interactions between fluorescence labeled proteins | Dr. G. Posthuma [2536548] | |
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| 9 High pressure freezing equipment (for fast fixing specimen for microscopy) | ||
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| 10 Immuno-electron microscopy | ||
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| 11 Immunofluorescence microscopy | ||
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| 12 JEOL 1010 | Dr. G. Posthuma [2536548] Transmission electron microscope max 100 KV | |
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13 JEOL 1200 EX
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Dr. G. Posthuma [2536548] Transmission electron microscope max 120 KV | |
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| 14 Leica TCS-NT | Dr. G. Posthuma [2536548] Confocal laser scanning microscope not suitable for life cell imaging. | |
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15 Live cell imaging (Fringe- GFP)
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Dr. G. Posthuma [2536548] | |
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| 16 Microtomes (ultrathin section, cryosection) | ||
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| 17 Transmission microscopy | Dr. G. Posthuma [2536548] | |
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18 Ultra cryo-microtomes (5)
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Cryosectioning of 50-1000 nm thick sections at temperatures ranging from - | |
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| 19 Ultra microtomes (3) | Routine plastic sectioning 20-1000 nm | |
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| 20 Ultraview | Dr. G. Posthuma [2536548] Spinning disk confocal microscope with climate chamber for life cell imaging | |
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| 21 Zeiss LSM 510 Meta | Dr. G. Posthuma [2536548] Confocal laser scanning microscope with climate chamber for life cell imaging | |
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