IB Project details
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| IB Project: Architecture and dynamics of the Cell | ||
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| Faculty: Science - Dept. of Biology | Department: Molecular Cell Biology | Click to see website |
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| IB Staff:
Dr. Paul van Bergen en Henegouwen p.vanbergen@uu.nl Tel: +31 (0)30 253 3349 |
PhD Student(s): MSc. Raimond Heukers r.heukers@uu.nl MSc. Marta Mania m.m.mania@uu.nl |
Postdoc(s): Dr. Mohammed El Khattabi m.elkhattabi@bio.uu.nl |
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| IB Staff:
Prof.dr. Johannes Boonstra j.boonstra@uu.nl Tel: +31 (0)30 253 3189 |
PhD Student(s): Drs. Maarten de Geest M.C.deGeest@uu.nl MSc. Milla Paalanen m.m.i.paalanen1@uu.nl |
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| IB Staff:
Dr. Fons Cremers a.f.m.cremers@uu.nl Tel: +31 (0)30 253 3002 |
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| IB Staff:
Dr. Willie Geerts w.j.c.geerts@uu.nl Tel: +31 (0)30 253 3449 |
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| IB Staff:
Dr. Wally Muller w.h.muller@uu.nl Tel: +31 (0)30 253 3588 |
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| IB Staff:
Dr. Jan Andries Post j.a.post@uu.nl Tel: +31 (0)30 253 3894 |
PhD Student(s): MSc. Vincent Krouwer v.j.d.krouwer@uu.nl |
Postdoc(s): Dr. Liesbeth Hekking e.h.p.hekking@bio.uu.nl Dr. Jord Stam j.c.stam@bio.uu.nl |
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| IB Staff:
Prof.dr. Theo Verrips c.t.verrips@uu.nl Tel: +31 (0)30 253 3421 |
PhD Student(s): MSc. Bram Dorresteijn b.dorresteijn@uu.nl MSc. Chris Emmerson c.d.emmerson@uu.nl MSc. Andrea Gorlani a.gorlani@uu.nl Drs. Alex Klarenbeek g.h.a.klarenbeek@uu.nl MSc. Nika Strokappe n.m.strokappe@uu.nl |
Postdoc(s): Dr. Anna Hultberg a.h.hultberg@uu.nl Dr. Lucy Rutten l.rutten@uu.nl |
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| Available techniques, facilities and expertise: 1 3D tomography |
Contact and or Remarks: |
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| 2 Antibodies specific for post-translational modifications of proteins & Mobility shift analysis on | Advantage: study processing and trafficking of proteins in cells | |
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| 3 Correlative live cell-immuno electron microscopy (CLEM) Advantage: combines advantages of LM and EM | ||
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| 4 Electron microscopy | ||
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5 EM facility
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SEM (dual beam SEM FIB) TEM | |
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| 6 EM tomography | To generate 3D images of cellular membrane structures. Cell Biology UMCU / Molecular Cell Biology. | |
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| 7 Fluorescence microscopy | ||
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| 8 Freeze-Fracture technique (EM) | ||
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| 9 High pressure freezing equipment (for fast fixing specimen for microscopy) | ||
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| 10 Immuno-electron microscopy | ||
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| 11 Immunofluorescence microscopy | ||
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| 12 Laser pressure catapulting (LPC) dissection | Advantage: for single cell | |
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| 13 Llama antibody technology | Selection of specific nanobodies, preparation of bivalent particles | |
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| 14 Microtomes (ultrathin section, cryosection) | ||
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| 15 Ni-coated vesicles | for Lipid protein interaction study | |
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| 16 Position Ablation Laser Microdissection (PALM) | ||
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17 Ultra cryo-microtomes (5)
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Cryosectioning of 50-1000 nm thick sections at temperatures ranging from - | |
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| 18 Ultra microtomes (3) | Routine plastic sectioning 20-1000 nm | |
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