IB Project details
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| IB Project: Membrane Enzymology | ||
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| Faculty: Science - Dept. of Chemistry | Department: Membrane Enzymology | Click to see website |
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| IB Staff:
Prof.dr. Dirk Bosch h.j.bosch@uu.nl Tel: +31 (0)30 253 3392 |
PhD Student(s): MSc. Berends Elsa e.berends@uu.nl (Shared with: L. Lugones co-supervisor) |
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| IB Staff:
Prof.dr. Maarten Egmond m.r.egmond@uu.nl Tel: +31 (0)30 253 3526 |
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| IB Staff:
Dr. Joost Holthuis j.c.holthuis@uu.nl Tel: +31 (0)30 253 6630 |
PhD Student(s): MSc. Radhakrishan Panatala Narendranath r.panatalanarendranath@uu.nl MSc. Hanka Paulitschke h.paulitschke@uu.nl MSc. Ana Vacaru-Hentes a.m.vacaru@chem.uu.nl |
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| IB Staff:
Dr. Toon de Kroon a.i.p.m.dekroon@uu.nl Tel: +31 (0)30 253 3424 |
PhD Student(s): Drs. Cedric De Smet c.h.desmet@chem.uu.nl |
Postdoc(s): Dr. Susanne Bryde s.bryde@chem.uu.nl Dr. Matthijs Kol M.A.Kol@uu.nl |
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| IB Staff:
(Director IB/IB contact) Prof.dr. Gerrit van Meer g.vanmeer@uu.nl Tel: +31 (0)30 253 3427 |
PhD Student(s): MSc. Rob Mesman r.j.mesman@uu.nl |
Postdoc(s): Dr. Per Haberkant p.haberkant@uu.nl |
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| Available techniques, facilities and expertise: 1 2D-TLC |
Contact and or Remarks: |
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2 Biochemistry
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depletion of fluorescence labeled lipids with BSA | |
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| 3 Biophysics | e.g. pulling tubes out of liposomes: study the relationship between lipid composition and curvature. | |
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| 4 CSLM | Dr. Dmitri Sakharov [2532852] | |
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| 5 Detergent-insolubility | raft? DRM? or artifact? | |
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| 6 Expression and purification of membrane proteins | ||
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| 7 FACS | Dr. Joost Holthuis j.c.holthuis@uu.nl Ing. Joep van Dikkenberg email. | |
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| 8 LC-MS analysis of lipids | ||
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| 9 Lipid transport and topology assays | ||
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| 10 Metabolic labelling techniques | ||
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| 11 Peptide Synthesis Facility | Prof.dr. M.R.Egmond email and Ing. R.C.Cox email. Peptides <30 AA with purity of 90% or higher can be delivered in 2 or 3 weeks. |
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| 12 Single molecule tracking | ||
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| 13 Sonication, gradients | by differential centrifugation | |
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| 14 Split ubiquitin assay | to study protein interactions (also membrane proteins) | |
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| 15 Subcellular fractioning | to localize a protein to a certain organelle | |
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| 16 Yeast genetics | ||
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| 17 Yeast-2-hybrid | to study protein interactions. Disadvantage: only non-membrane bound proteins | |
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